P-15: Morphometrical and Histometrical Study of Protective Effect of Hydro Alcoholic Extract of Lepidium Sativum Seeds on Testis in Normal and Diabetic Male Rat

Background: Clinical as well as experimental studies revealed impairment of spermatogenesis in diabetes. Diabetes leads to cell death due to increased oxidative stress. Lepidium sativum (LS) is a plant with antidiabetic effect.The aim of this study was to investigate the protective effect of LS on sperm parameter and testis morphology in adult male rat. Materials and Methods: In this study male adult rat were divided into six groups. The control group received normal saline, Groups 2 and 3 received 50 and100 mg/kg LS mg/ kg respectively. Group 4 received a single dose of 65 mg/ kg STZ for induction of diabetes. Groups 5and6 received 50 and100 mg/kg LS respectively plus a single dose 65 mg/kg STZ. LS extract and normal saline was administered for 21 days. Animals were sacrificed 22 days after treatment and evaluations were made by determining of sperm count and sperm quality, histological study of testis and measuring of plasma testosterone level. Statistical analysis was performed using ANOVA and Tuckey test. Results: LS -treated groups showed a significant decrease in the number of germinal cells of seminiferous duct (p<0.01) and sperm motility (p<0.01) compared with controls. Number of germ cells was reduced in LS -treated group and there was a significant difference with control groups. In both 50 and 100 mg/kg treated group there were no significant differences in morphologic and morphometric studies. Conclusion: Administration of Lepidium sativum in doses of 50 and 100 mg/kg for 21 days has toxic effect on sperm parameter and morphology of testis.